Regulatory

Part:BBa_K2934002:Design

Designed by: Shira Levi   Group: iGEM19_Technion-Israel   (2019-10-10)


pKatA promoter for Bacillus subtilis


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    COMPATIBLE WITH RFC[21]
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

Suitable for Bacillus subtilis.

Primers for isolation of the gene (with RFC[10] suffix and prefix):

fwd: 5'-GGCCGCTTCTAGAGATAACTATTTT-3'

rev: 5'-CGCTACTAGTATAAGAACATAATATCATCTC-3'

Source

The sequence was taken from Herbig et al. 2001 [1].

References

[1] Andrew F. Herbig and John D. Helmann. 2001. Roles of metal ions and hydrogen peroxide in modulating the interaction of the Bacillus subtilis PerR peroxide regulon repressor with operator DNA, Molecular Microbiology.